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RNAi & MicroRNA
Rosetta Genomics: Unrecognized and Undervalued PDF Print E-mail
Wednesday, 10 March 2010 18:00

MicroRNAs are naturally produced, using instructions encoded in DNA and are used in regulating protein production. Rosetta Genomics Ltd. (NasdaqGM: ROSG) is a leading developer of microRNA-based molecular diagnostics.

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New Research May Help Predict Post-Surgery Survival Rate For Mesothelioma Patients PDF Print E-mail
Wednesday, 10 March 2010 16:04

Until now, doctors have had difficulty determining which mesothelioma patients were the best candidates to receive the full array of anti-cancer treatments including surgery, chemotherapy and radiation. But new research may give doctors one more tool for identifying those patients, a New York personal injury lawyer said.

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Hutch Lab Improves Protocol for qRT-PCR Analysis of microRNA Biomarkers from Blood PDF Print E-mail
Monday, 08 March 2010 18:57

Researchers from the Fred Hutchinson Cancer Research Center have developed and published an improved protocol for using quantitative reverse transcription PCR to analyze circulating microRNA biomarkers in blood plasma and serum.

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Researchers Suggest New RNAi Method to Identify Genes Predictive of Paclitaxel Response PDF Print E-mail
Friday, 05 March 2010 18:14

Scientists at Cancer Research UK’s London Research Institute (LRI) and the Technical University of Denmark have developed an RNAi-based approach to determine paclitaxel response. Focusing on estrogen-receptor (ER)/progesterone-receptor (PR)/human epidermal growth factor receptor 2 (HER2; ERBB2)-negative (triple-negative) disease, they sequentially deleted 829 genes involved in cells’ response to the chemotherapy to see which missing or faulty genes would prevent the drug from working.

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MicroRNAs of Bombyx Mori Identified by Solexa Sequencing PDF Print E-mail
Friday, 05 March 2010 16:09
MicroRNA (miRNA) and other small regulatory RNAs contribute to the modulation of a large number of cellular processes. We sequenced three small RNA libraries prepared from the whole body, and the anterior-middle and posterior silk glands of Bombyx mori, with a view to expanding the repertoire of silkworm miRNAs and exploring transcriptional differences in miRNAs between segments of the silk gland.
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Febit's miRBase 14 Geniom-Biochip Now With 58 Additional New Sequences Available for Cancer Research PDF Print E-mail
Wednesday, 03 March 2010 15:32
Febit today announced that an updated version of  the company's Geniom Biochip containing 58 new discovered sequences in addition to all of the Homo sapiens microRNAs (miRNA) from miRBase version 14 (www.mirbase.org) is now available for cancer research.
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Gene-Based Stem Cell Therapy Specifically Removes Cell Receptor That Attracts HIV PDF Print E-mail
Monday, 01 March 2010 14:58
UCLA AIDS Institute researchers successfully removed CCR5 -- a cell receptor to which HIV-1 binds for infection but which the human body does not need -- from human cells. Individuals who naturally lack the CCR5 receptor have been found to be essentially resistant to HIV.
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Dynamical Modeling of MicroRNA Action On The Protein Translation Process PDF Print E-mail
Monday, 01 March 2010 12:27
Protein translation is a multistep process which can be represented as a cascade of biochemical reactions (initiation, ribosome assembly, elongation, etc.), the rate of which can be regulated by small non-coding microRNAs through multiple mechanisms.
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MicroRNA causes "double whammy" of metastasis and angiogenesis in breast cancer PDF Print E-mail
Friday, 26 February 2010 22:46

Primary tumours are not the big killers, it's the secondary tumours that account for 90 per cent of the mortality of cancer.

Now a new study out of the U.S. has shown that the microRNA, miR-9, plays a significant role in metastasis, both enabling cells to spread to other parts of the body as well as encouraging tumour angiogenesis.

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Melanoma Transcriptome Reveals Novel Genomic Alterations Not Seen Before PDF Print E-mail
Wednesday, 24 February 2010 16:12
Melanoma, the most deadly form of skin cancer, afflicts more than 50,000 people in the United States annually and the incidence rate continues to rise. In a study published online in Genome Research, scientists have delved deeper than ever before into the RNA world of the melanoma tumor and identified genomic alterations that could play a role in the disease.
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Unified Translation Repression Mechanism For MicroRNAs And Upstream AUGs PDF Print E-mail
Wednesday, 10 March 2010 17:02
MicroRNAs (miRNAs) are endogenous small RNAs that modulate gene expression at the post-transcriptional level by binding complementary sites in the 3'-UTR. In a recent genome-wide study reporting a new miRNA target class (miBridge), we identified and validated interactions between 5'-UTRs and miRNAs.
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DNA-PKcs Plays A Dominant Role In The Regulation Of H2AX Phosphorylation In Response To DNA Damage And Cell Cycle Progression PDF Print E-mail
Monday, 08 March 2010 19:01
When DNA double-strand breaks (DSB) are induced by ionizing radiation (IR) in cells, histone H2AX is quickly phosphorylated into gamma-H2AX (p-S139) around the DSB site. The necessity of DNA-PKcs in regulating the phosphorylation of H2AX in response to DNA damage and cell cycle progression was investigated.
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Study Identifies Key Cause of Chronic Leukemia Progression PDF Print E-mail
Monday, 08 March 2010 17:53
Researchers have discovered a key reason why a form of leukemia progresses from its more-treatable chronic phase to a life-threatening phase called blast crisis.
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Detection of siRNA induced mRNA Silencing by RT-qPCR: Considerations for Experimental Design PDF Print E-mail
Friday, 05 March 2010 17:10
RNA interference (RNAi) has been one of the most rapidly expanding areas of biological research in the past decade, revolutionizing the ability to analyze gene function. Thorough validation of siRNA duplexes is required prior to use in experimental systems, ideally by western blotting to show a reduction in protein levels.
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Altered Expression of MicroRNAs in the Myocardium of Rats With Acute Myocardial Infarction PDF Print E-mail
Wednesday, 03 March 2010 16:34
MicroRNAs are important cellular components and their dysfunctions are associated with various disease. Acute myocardial infarction (AMI) is one of the most serious cardiovascular diseases.

Although several miRNAs have been reported to be associated with AMI, more novel miRNAs are needed to be investigated to ascertain if they are associated with AMI.
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Key Player Found for a Cancer Typical in Down Syndrome PDF Print E-mail
Wednesday, 03 March 2010 14:57
Between 5 and 10 percent of babies with Down syndrome develop a transient form of leukemia that usually resolves on its own. However, for reasons that haven't been clear, 20 to 30 percent of these babies progress to a more serious leukemia known as Down syndrome acute megakaryoblastic leukemia (DS-AMKL), which affects the blood progenitor cells that form red blood cells and platelets.
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How A MicroRNA May Help Spread Breast Cancer PDF Print E-mail
Monday, 01 March 2010 13:24

US researchers have shown how a microRNA, a short RNA molecule that regulates the activity of genes, called miR-9 may be important in a breast tumours’ ability to spread to other parts of the body and gather nutrients to survive and grow.

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Regulus, GSK Ink Second miRNA Rx Collaboration PDF Print E-mail
Friday, 26 February 2010 22:58

Regulus Therapeutics said this week that it has formed a new collaboration with British drugs giant GlaxoSmithKline to develop drugs targeting microRNA-122, with hepatitis C infection as the lead indication for the effort.

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Epigenetics Makes Cancer Cell "Lean Mean Machine" PDF Print E-mail
Friday, 26 February 2010 21:53

Researchers led by scientists from the Garvan Institute of Medical Research in Sydney have found some surprises in the epigenome of prostate cancer.

They found vast regions of the genome are silenced, more than previously expected, allowing the cancer cell to focus on proliferation to the exclusion of other functions.

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Oral Cancer Study Shows Full Tumor Genome; Novel Method Speeds Analysis for Individualized Medicine PDF Print E-mail
Wednesday, 24 February 2010 15:17
Mayo Clinic researchers along with collaborators from Life Technologies are reporting on the application of a new approach for sequencing RNA to study cancer tumors. Their findings from a proof-of-principle study on oral carcinomas appear in the current issue of PLoS ONE, the online science journal.
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